dextran hydrodynamic radius dextran hydrodynamic radius

Increased TEWL values are evidence of micropores formation. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Journal of American Chemical Society, 76 (20), 5041-5052. Molecular weight, molecular weight distribution and molecular size of a native dextran. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Intrinsic viscosity molecular weight relationship. Citation-(RIS The skin poration was performed using the microporation device (LG electronics, Korea). Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Clipboard, Search History, and several other advanced features are temporarily unavailable. What is hydrodynamic radius Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Learn More. dextran The https:// ensures that you are connecting to the Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. dextran hydrodynamic radius. Polymer molecular weight distribution. Formul. CAS Journal of Physical Chemistry. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Eremeeva, T. E.; Bykova, T. O. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). 2019;26(1):25261. WebR H Hydrodynamic radius [m] The basic DLS setup. Arond L.H. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Hydrodynamic Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. 20K groups (n=3, *: p<0.05, **: p<0.01). The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Biomaterials, 22, 1891-1898. Expert Opin Drug Deliv. Ther. Fluorescent and Biotinylated DextransSection 14.5 Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. The above data are not useful because it can not be compared with those obtained in this work. Dextran Fractions. Learn More. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. (2008). 2000;3(9):31826. 113, 81-86. dextran The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Harding, Stephen E. (1997). Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. (2003). Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Biophysical Journal, 27, 177-181. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. 2006;3(3):37385. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Ind. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Manage cookies/Do not sell my data we use in the preference centre. Macromolecules, 33, 5730-5739. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Figure4 shows the histological analysis of micropores in human cadaver skin. Dextran Ioan, C.E. Front Microbiol. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. ; Grishchenko, A.E. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Synthesis and characterization of blue dextrans The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Radiofrequency with microneedling. Polyelectrolytes as secondary calibration standards for aqueous SEC. De Belder, A.N. Journal of Applied Polymer Science, 72, 871-876. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Dextran 670 GPC Standard | Pharmacosmos Dextran Biomacromolecules 8, 2464-2475. Food Funct. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). J. @ Nilimesh See attached. where tr is the column retention time in minutes (2 of 0.9792). There is a deviation from linearity after 580kDa [69, 70]. Advances in Cosmetic Surgery. Microporation was performed 2 times on each sample. hydrodynamic The unfolding of iRFP713 in a crowded milieu - PeerJ The authors declare that they have no competing interests. Journal of Controlled Release, 119, 5-24. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Polysaccharide hydrogels for modified release formulations. (1958). One day before the experiment, the skin was stabilized in the skin culture medium. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. American Journal of Food Science and Technology 2013, 1(3), 60-66. PubMedGoogle Scholar. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. eCollection 2018 Feb. Molecules. 10K, and c M.W. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Exp Cell Res. Dextrans can function efficiently as anterograde or retrograde tracers, Dextrans in Aqueous Solution. Experimental Review on Intrinsic The Mark-Houwink a constant is close to 0.5 in poor solvents. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Dilute solution properties and degree of chain branching WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Please enable it to take advantage of the complete set of features! 2012;101(8):28619. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Structure Properties of Dextran. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Colloid Polym. FITC-dextran solution was prepared at 1mM in PBS. Journal of Colloid Science, 31, 308-328. WebFITC Products. The human skins were from the back or thigh of a 24-year-old woman. Ahn, G.Y., Eo, HS., Kim, D. et al. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. eCollection 2015. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. Treatment of acne scars by fractional bipolar radiofrequency energy. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. 1905/05) for their financial support. Google Scholar. Gekko, K. (1971). Beer, M.U. pp13-21. 2003;10:33847. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. Huggins equation [41] gives the relation to the relative viscosity increase (i). Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Disclaimer. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. 2. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Sci. Solution properties of branched dextran. 43-1, 115-147. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. FOIA osmotic pressure or light scattering). Experimental and numerical study on the mixed lubrication This study was supported by the LG Electronics. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). 2009;301(8):60913. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. (1954). Unable to load your collection due to an error, Unable to load your delegates due to an error. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. HHS Vulnerability Disclosure, Help Soc., 16, 3A, 426-433. HSE and DK helped to porate the skin and to measure TEWL. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. Intrinsic viscosity of bovine serum albumin conformers. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Principles of Size Exclusion Chromatography SEC (GFC The enhanced permeability through micropores was confirmed by Franz diffusion cell. ; Tsuchiya, H.M.; Rist, C.E. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. 2009;8(2):1739. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. and transmitted securely. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Eng. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. De Belder, A. N. (2001). Harding, S. E., & Simpkin, N. J. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. ; Wood, P.J. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. 2008;28(5):64557. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Drug deliv. dextran Correspondence to ; van Nostrum, C.F. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Huggins, Maurice L. (1942). Advances in Physical Chemistry, Vol. FITC-dextran solution (600L) was added to the donor chamber. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399.