Alexa Fluor® FluoroNanogold™ is a unique, dual-purpose probe. Designed to label 1 mg of IgG; Simple protocol — react, separate, use; Stabilizing proteins must be removed from the sample before labeling The Benefits of Alexa Fluor™ Dyes Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. Experimental Protocols for Selective Plasma Membrane Labeling Labeling Live Eukaryotic Cells This is a general procedure for labeling live, cultured cells that are adhering to coverslips. Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line.For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. View our protocol for Staining Membrane-associated Proteins. 10 ug) and large quantities of primary antibodies with ease. Store at 2°C to 8°C, protected from light. Catalog number. BP Fluor 488 (A max 493 nm, E max Label (Ex/Em): Alexa Fluor® 488 (~495/519 nm). Alexa Fluor® 488 Protein Labeling Kit | 4 3.3 Calculate the degree of labeling: moles dye per mole protein = A 494 × dilution factor where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. Secondary antibodies conjugated with Alexa Fluor ®647 (Invitrogen), ATTO 488 (Jackson Immuno), and Alexa Fluor 568 (Invitrogen) are recommended. 的 兔 Plexin A3 抗体. Answered by: Tech Support Europe. Samples were continuously illuminated, and images were collected every 5 seconds with a cooled CCD camera. Normal mouse IgG1 Alexa Fluor® 488: sc-3890 is suitable as an unspecific negative control in any experiment with an Alexa Fluor® 488-conjugated IgG1 primary antibody. Following incubation of the primary and secondary antibodies, Alexa Fluor ® 488 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to the cells. Product overview Streptavidin Alexa Fluor ® 488 Monovalent Antibody Labeling Kit ab272187 is used to label biotinylated antibodies with Alexa Fluor ® 488. 488 (ebAF488, 1:800). modern browser such Google Chrome Hello. Spin filter. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. PROTOCOL; Protocol Spot-Label Alexa Fluor 488 (PDF) BROCHURE; Product brochure (PDF) ×. Immediately transfer Labeled Tubulin-Alexa Fluor® 488 to -80°C upon receipt. ALEXA FLUOR® 488. In vitro Protein Labeling: Reaction of SNAP-Sur-face Alexa Fluor 488 (10 µM) with purified SNAP-tag protein (5 µM) in vitro, followed by mass spec analysis, indicated an efficiency of labeling of 95%. 3% BSA in PBS for 1 hr at RT, and then incubated with Invitrogen Alexa Fluor Plus 488 goat anti-mouse IgG secondary antibody (Product # A32723) at a dilution of 1: 1000 for 1 hr at RT. Alexa Fluor™ 488 Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns and collections tubes. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all . abberior STAR 580. . Because of its optimal photoswitching properties, Alexa Fluor®647 should be used when staining for only . improving abcam.com and welcome your feedback. Product information: Sema4C Antibody ALEXA FLUOR 488 - Gentaur.com - Product info. Alexa Fluor . The Alexa Fluor 488 Protein Labeling Kit provides the materials needed to conveniently label an antibody or protein with amine-reactive green fluorescent Alexa Fluor 488 dye (ex/em 495/519 nm). This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 488. Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm) . Spectrally similar to fluorescein, but the signal is less pH-dependent and more photostable. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9181G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). Reconstitution Calculator. Surface Alexa Fluor 488 was determined to be 93% by HPLC analysis. modern browser such Google Chrome Hello. For IgGs, we find that labeling with 4-9 moles of Alexa Fluor® 488 dye per Immediately transfer Labeled Tubulin-Alexa Fluor® 488 to -80°C upon receipt. 5.7 Excitation and emission peaks for the four labels that are available in different kits: - (ab272187) Alexa Fluor® 488: Ex 495 nm, Em 520 nm - (ab272188) Atto542 : Ex 542 nm, Em 562 nm These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. improving abcam.com and welcome your feedback. Once fixed, please follow CST protocol for immunostaining. improving abcam.com and welcome your feedback. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. 电话: +1 877 302 8632 传真: +1 888 205 9894 (Toll-free) 电子邮件: orders@antibodies-online.cn S34253 SelectFX ® Alexa Fluor 488 Endoplasmic Reticulum Labeling Kit (S34200) 2-6°C Components Anti-protein disulfide isomerase (PDI), mouse IgG2b* 50 µL 500 µg/mL solution in PBS, 2 mM sodium azide 2-6˚C Protect from light DO NOT FREEZE • • • Components stable for up to 3 months. bs-1123R-A488. Recommended dilution for GFP-Booster Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647 IF/ICC/IHC: 1:500- 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 . Date published: 2020-06-23. Alexa Fluor™ 488 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 - pH 值 10）。 除反应性染料制剂外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 Alexa Fluor™ 488 染料 . With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal. Please read the entire protocol before starting. 3. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled primary antibodies with the Zenon™ mouse IgG 1 labeling kits. Alexa Fluor®568 can also be substituted with Cy™ 3B when a yellow-absorbing dye is needed. The image contains overlay of GFAP (green) and nuclei (blue). It is suitable for standard fluorescein filter sets. Sema4C Antibody, ALEXA FLUOR 488. CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. Thermo Fisher alexa fluor 488 647 protein labeling kit Molecular Probes Protein Labeling Kits provide a convenient means for attaching a fluorescent label or biotin to an antibody or a protein larger than 40 kDa Conjugates are ideal for multiple applications including flow cytometry fluorescent microscopy immunohistochemistry primary detection ELISAs immunocytochemistry FISH and more Kits are . Alexa Fluor® 488 Conjugation Kit - Lightning-Link® (ab236553) provides a simple and rapid procedure to covalent labeling of antibodies with Alexa Fluor® 488 by targeting primary amine groups (e.g. Prepare the proteins • For optimal labeling efficiency, the purified protein must be in a buffer free of ammonium ions or primary amines. Once the labeled tubulin is approximately halfway thawed, remove from the water bath and thaw to completion with gentle flicking. Conjugated Primary Antibodies. Have you used Rabbit IgG Alexa Fluor® 488-conjugated Antibody? Alexa Fluor™ C2 647 maleimide] IMPORTANT DISCLAIMER: The following is a sample protocol for labeling targets with AF647 maleimide [equivalent to Alexa Fluor™ C2 647 maleimide]. Bioss Primary Conjugated Antibodies. 4. Right: Control staining of untransfected cells. modern browser such Google Chrome Hello. Important Features of Protein Labeling Kits: Labeled proteins typically ready to use in 2 hr (~30 min hands-on time) Features. improving abcam.com and welcome your feedback. improving abcam.com and welcome your feedback. The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. You can use gel filtration as well as dialysis. Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6127G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). Alexa Fluor 488 Microscale Protein Labeling Kit, supplied by Thermo Fisher, used in various techniques. Product Description. . Additionally . The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Take look. Take look Maybe later Hello. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. -20℃ for 24 months , avoid repeated freezing and thawing. The Alexa Fluor 647 reactive Human Fibrinogen Conjugate Specifications: 1. BP Fluor 488 (Alexa Fluor ® 488 equivalent) has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Cy2, Alexa Fluor 488, FITC in many applications. Cy5. Label. ALEXA FLUOR 488. Conjugated with. lysines). Alternative name. Thaw only when ready to use by placing briefly in a 37°C water bath. 5.6 Do not combine Streptavidin Alexa Fluor® 488 (Monovalent) with protocols where standard streptavidin is used. SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit 2-6˚C Components Anti-peroxisomal membrane protein 70 (PMP 70) rabbit IgG fraction* (Component A) 50 µL 250 µg/mL solution in PBS, 2 mM sodium azide • 2-6˚C • Protect from light • DO NOT FREEZE When stored as directed, the components are stable for up to 3 months. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated TCF1/TCF7 (C63D9) Rabbit mAb #2203. In some cases, expression of the FOS gene has also . This includes flow cytometry and immunofluorescence. I want to track its localisation in cell by labeling it with a succinimidyl ester of Alexa fluor 488. Alexa Fluor ® 488 Microscale Protein Labeling Kit 3 Figure . Take look. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. Storage and Handling. It can also be used to conjugate other proteins or peptides. Each kit provides the components needed to perform three protein conjugations and purifications. ATTO 633. Modification. Streptavidin Alexa Fluor ® 488 Monovalent Antibody Labeling Kit ab272187 is used to label biotinylated antibodies with Alexa Fluor ® 488.. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Take look Maybe later Hello. ALEXA FLUOR 488. Images were taken on a The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to fluorescein). With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical . Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. Labeling reaction using BG-Alexa Fluor ® 488 in Solution Introduction. Alexa Fluor® 488 FluoroNanogold™ reagents consist of affinity-purified Fab' fragments conjugated to both Alexa Fluor® 488 dye (green) and the 1.4 nm Nanogold® particle.1 An Alexa Fluor® 488 FluoroNanogold™ Fab' conjugate is shown in Figure 1. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X . However, it carries multiple negative charges, which can significantly reduce conjugates aggregation in the bio application. It probably recognizes the R-G-D peptide in C3b. Alexa Fluor ® 488, Alexa Fluor ® 568, Alexa Fluor ® 647, ATTO488, ATTO594, ATTO647N, unconjugated. . Home Protocols Labeling reaction using BG-Alexa Fluor ® 488 in Solution. This protocol only provides a guideline and should be modified according to your experimental needs. Labeling protocol. Lightning-Link allows for conjugation of antibodies and proteins in less than 20 minutes, using a very simple protocol which is free of separation steps, saving researchers time and precious materials. Alexa 488 absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell and tissue staining of multiple proteins with Alexa Fluor ® 594 and Alexa Fluor ® 647 conjugated secondary antibodies. Bioz Stars score: 94/100, based on 1 PubMed citations. The Antibody - Streptavidin Alexa Fluor ® 488 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.. Several antibodies can be labeled with this kit, and its associated kits, and . Figures Please ensure that your instrument's configurations (lasers and optical filters) are appropriate for this dye. ALEXA FLUOR. Alexa Fluor 647. ZERO BIAS - scores, article reviews, protocol conditions and more Scale bar, 10 μm. Once fixed, please follow CST protocol for immunostaining. Take look. The Antibody - Streptavidin Alexa Fluor ® 488 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes. Alexa Fluor ® 488 Conjugation Kit / Alexa Fluor ® 488 Labeling Kit ab236553 uses a simple and quick process for Alexa Fluor 488 labeling / conjugation of antibodies. Alternative name. Lyophilized product can be dissolved in buffer (e.g., sodium bicarbonate, pH 8.3) for use. Label. Left: HeLa cells transiently expressing Spot-Tag?-Lamin B1 were immunostained with Spot-Label?